Detection of rabies-specific antigens by egg yolk antibody (IgY) to the recombinant rabies virus proteins produced in Escherichia coli.

نویسندگان

  • Yurie Motoi
  • Satoshi Inoue
  • Hajime Hatta
  • Kozue Sato
  • Kinjiro Morimoto
  • Akio Yamada
چکیده

We obtained rabies-specific egg yolk antibodies (IgY) by immunizing hens with recombinant His-tagged nucleoprotein and phosphoprotein (rN, rP) of the rabies virus (CVS-11 strain) expressed in Escherichia coli. The anti-rN and rP IgY were shown to bind specifically to the respective proteins of the CVS-11 strain of rabies virus by Western blotting, immune fluorescent assay and immunohistochemistry, indicating that IgY to rabies recombinant proteins could serve as a reagent for diagnosis of rabies virus infection.

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عنوان ژورنال:
  • Japanese journal of infectious diseases

دوره 58 2  شماره 

صفحات  -

تاریخ انتشار 2005